FIGURE 10.

Up-regulation of WBSCR17 mRNA and O-glycosylation in response to concentration of GlcNAc. The expression of WBSCR17 was examined by RT-PCR (A) and real-time PCR (B) in the HEK 293T cells that were treated with or without a high concentration of GlcNAc. The treatment with GlcNAc up-regulated WBSCR17 mRNA. Data shown represent means ± S.D. (error bars) of three independent experiments. C, ABA lectin blot analysis of glycoproteins in the cell lysates. The GlcNAc treatment enhanced the expression of ABA-positive proteins with relatively high molecular weight as indicated by arrowheads (left). Total proteins were stained with Coomassie Brilliant Blue (CBB) (right). D, a high concentration of GlcNAc inhibited proliferation and fluid phase endocytosis. The bottom panels show incorporated high molecular weight dextran through macropinocytosis. E, a hypothetical scheme of the role of WBSCR17. The expression of WBSCR17 corresponds to the nutrient concentration as exemplified by environmental GlcNAc and may work as a modulator of nutrient uptake through controlling macropinocytosis in the cells.