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. 2012 Jul 11;287(38):32222–32235. doi: 10.1074/jbc.M112.370932

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Purification and enzyme assay of WBSCR17. Recombinant soluble WBSCR17 was purified with anti-FLAG-M2 agarose. A, purified WBSCR17 was subjected to SDS-PAGE and silver-stained. An arrowhead indicates purified recombinant WBSCR17. B, Western blotting of purified recombinant WBSCR17 with an anti-FLAG antibody. C, assay for the enzymatic activity of purified WBSCR17 using several mucin peptides as acceptor substrates. WBSCR17 exhibited very low but detectable activity toward MUC5AC-1, MUC5AC-2, and MUC7 peptides. Data shown represent means ± S.D. (error bars) of three independent experiments.