FIGURE 7.
WBSCR17 regulates fluid phase endocytosis. A, labeling with fluorescein-conjugated dextran Mr 3,000. The cells were labeled with Mr 3,000 dextran to visualize the fluid phase endocytosis. Fluorescence microscopic observation revealed large vesicles containing dextran in the WBS17KD cells. The bottom panels are magnified images of the boxed areas in the top panels. B and C, flow cytometric analysis of cells labeled with Mr 3,000 (B) and Mr 10,000 (C) dextran. Black lines, incorporation at 37 °C; gray areas, incorporation at 4 °C; black areas, without dextran. Histograms are representative of three independent experiments. D and E, fluorescence intensity of the cells as determined by flow cytometry. Data shown represent means ± S.D. (error bars) of three independent experiments. *, p < 0.05. D, knocking down WBSCR17 enhanced uptake of Mr 10,000 dextran but not that of Mr 3,000 dextran. E, overexpression of WBSCR17 inhibited incorporation of Mr 10,000 dextran and rescued the effect of WBSCR17 siRNA.