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Western analysis using 25 μg of protein of whole cell extracts of rat, mouse, and human islets for basal and IL-1β-stimulated levels of COXs and PGESs. COX-1 protein levels did not increase with IL-1β, whereas COX-2 protein levels did. cPGES and mPGES-2 protein levels were present in the nonstimulated state, and neither was stimulated by IL-1β. mPGES-1 was not detectable with or without stimulation by IL-1β.
