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. 2012 Jul 20;287(38):32246–32253. doi: 10.1074/jbc.M112.364612

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Western analyses for mPGES-1 using microsomal versus whole cell extracts. Top panel, rat islets are shown. Whole cell extracts and microsomal extracts using greater amounts of protein than were used in Fig. 3 failed to reveal stimulation of mPGES-1 levels by IL-1β. Bottom panel, human islets are shown. mPGES-1 was detectable in whole cell extracts using greater amounts of protein than were used in Fig. 3 and revealed mPGES-1, but these levels were not increased when islets were treated with IL-1β.