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Knockdown of p35 by siRNA reduces HuR/Cdk5 interaction and provokes amplification of centrosome number. A, reduction of p35 expression by siRNA (a) and reduction of Cdk5 co-immunoprecipitation with HuR (b). B, illustration of amplification of centrosome number per nuclei following p35 knockdown. Nuclei were stained with DAPI, and centrosomes were marked with the PACT domain of pericentrin attached to the red fluorescence protein. Bar, 50 μm. IP, immunoprecipitation; IB, immunoblot.
