Table 2. Number of RNAi Genes in Selected Fungal Species.
| RNAi Gene | Ascomycetes |
Basidiomycetes |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Nc | Ca | Sc | Sp | Pc | Cc | Lb | Cn | Mg | Pgta | Sr | Uh | Um | |
| Ago | 2 | 1 | 0 | 1 | 7 | 8 | 6b | 2 | 0 | 3 | 1 | 1 | 0 |
| Dicer | 2 | 0 | 0 | 1 | 3 | 3 | 2 | 2 | 0 | 3 | 1 | 1 | 0 |
| RdRP | 3 | 0 | 0 | 1 | 9 | 7 | 6b | 1 | 0 | 5 | 3 | 3 | 0 |
Ca, Candida albicans; Cc, Coprinopsis cinerea; Cn, C. neoformans; Lb, L. bicolor; Mg, M. globosa; Nc, N. crassa; Pc, Phanerochaete chrysosporium; Pgt, P. graminis f. sp tritici; Sc, S. cerevisiae; Sp, S. pombe; Sr, S. reilianum; Uh, U. hordei; Um, U. maydis. Table adapted from Nakayashiki et al. (2006). Note that the number of genes is reduced in the pathogenic basidiomycetes compared to other basidiomycetes.
Comparative BLAST searches identified one more possible (partial) Ago sequence and two more matching Dicer sequences in the currently available Pgt genome (Duplessis et al., 2011; http://www.broadinstitute.org/annotation/genome/puccinia_group/MultiHome.html; March, 2012); comparative phylogenetic analysis suggests the likely presence of allelic variants in the available genome information that is derived from dikaryotic material. Fewer genes were found in the currently available Pt genome that is likely still partial: one Ago, four Dicer, and two RdRp sequences. Even fewer were found in the partial Pst genome sequence currently available (Cantu et al., 2011).
Database: Laccaria bicolor Best Models (proteins) Joint Genome Institute.