Figure 7.

Activity of the CYC Binding Site in Activating a GUS Reporter Gene in Transient Gene Expression Assays.

(A) The GUS reporter gene plasmids are constructed by inserting tetramer of the CYC binding sites in the CYC1C promoter, either wild-type (cbs1) or mutated (mcbs1), into the upstream of 35S minimal promoter. The pCAMBIA1302 and 35S:CYC1C plasmids are used as a negative control and an effector plasmid, respectively.

(B) The sequence cbs1 activates the GUS reporter gene in the presence of CYC1C. The GUS activity is examined with 4-methylumbelliferyl-β-d-glucuronide. The data shown (mean ± sd) are the average of three experiments.