Figure 3.

ACHT1 Reduction Requires Photosynthetic Reducing Equivalents.

Immunoblot assay showing the in vivo oxidized state of ACHT1 active site Cys residues captured in plants that were left in the dark (A) or illuminated but treated with 50 μM of the photosynthetic transport inhibitor DCMU (B). Equal protein loading was verified as in Figure 2. The results shown are representative of three independent experiments.