Figure 5.

2-Cys Prx Is Reduced by ACHT1 in Planta.

Immunoblot assay showing the oxidized state of 2-Cys Prx Cys residues in plants after 5 min of illumination. The number of oxidized Cys residues (ox-Cys) was determined by the increased molecular mass of 2-Cys Prx after labeling with mPEG (indicated by arrows) as detected by 2-Cys Prx-specific sera. The assay showed that the 2-Cys Prx pool was more reduced in plants with increased expression of ACHT1 (OE) relative to wild-type plants (WT). Equal protein loading was verified as in Figure 2. The results shown are representative of three independent experiments. RBCL, ribulose-1,5-bis-phosphate carboxylase/oxygenase.