Figure 3.

Description of flv Inactivation Mutants and the Expression of the Operon flv4 -flv2 at Air Level of CO₂.

(A) Schematic description of the four flv inactivation mutants mostly used in this study and the location of the kanamycin- (Km), hygromycin- (Hyg), or spectinomycin (Spc)-resistant cassette at different positions.

(B) Transcript accumulation of sll0217-19 in the wild type (WT) and the three flv mutants analyzed by real-time quantitative RT-PCR. Transcription abundance is shown as relative units (r.u.). The transcript level of the rnpB gene is used as a reference. The results are the mean from three independent experiments ± sd.

(C) Protein immunoblot demonstrating the protein accumulation of Flv4, Sll0218, and Flv2 in the total cell extract from wild type and the four flv mutants.

[See online article for color version of this figure.]