Table 3. Kinetic Data of Flash-Induced Fluorescence Relaxation Components in the Wild Type and Δflv4 Mutant Grown at High CO2, Air Level of CO2, and 48 h after a Shift from High to Air Level of CO2.
| Strains and Growth Conditions | Fast |
Middle |
Slow |
|||
|---|---|---|---|---|---|---|
| T1 (ms) | A1 (%) | T2 (ms) | A2 (%) | T3 (s) | A3 (%) | |
| High CO2 |
No addition |
|||||
| The wild type | 0.476 ± 0.017 | 58 ± 1.5 | 3.6 ± 0.29 | 26 ± 1.6 | 17.7 ± 1.2 | 16 ± 0.3 |
| Δflv4 | 0.469 ± 0.007 | 59 ± 1.7 | 3.7 ± 0.15 | 24 ± 1.9 | 14.7 ± 1.2 | 17 ± 0.5 |
| + DBMIB | ||||||
| The wild type | 1.360 ± 0.020 | 44 ± 1.5 | 33.6 ± 0.3 | 36 ± 1.7 | 4.3 ± 0.7 | 20 ± 0.2 |
| Δflv4 | 1.310 ± 0.040 | 45 ± 1.8 | 36.5 ± 0.3 | 35 ± 2.3 | 3.6 ± 0.7 | 20 ± 0.5 |
| Shift to air (48 h) | No addition | |||||
| The wild type | 0.503 ± 0.010 | 64 ± 1.6 | 4.3 ± 0.1 | 20 ± 0.7 | 11.6 ± 3.7 | 16 ± 0.9 |
| Δflv4 | 0.548 ± 0.045 | 49 ± 2.7 | 3.1 ± 0.5 | 30 ± 1.7 | 12.4 ± 2.8 | 21 ± 0.5 |
| + DBMIB | ||||||
| The wild type | 1.015 ± 0.030 | 52 ± 0.3 | 30.6 ± 2.7 | 30 ± 0.6 | 4.7 ± 0.9 | 18 ± 0.3 |
| Δflv4 | 1.430 ± 0.015 | 40 ± 1.3 | 39.8 ± 0.6 | 35 ± 2.1 | 4.0 ± 0.6 | 25 ± 0.9 |
| Air (growth) | No addition | |||||
| The wild type | 0.643 ± 0.025 | 65 ± 0.5 | 6.8 ± 0.4 | 19 ± 0.2 | 8.4 ± 1.1 | 16 ± 0.4 |
| Δflv4 | 0.822 ± 0.028 | 48 ± 2.1 | 7.7 ± 0.3 | 25 ± 1.6 | 3.5 ± 0.2 | 27 ± 1.3 |
| + DBMIB | ||||||
| The wild type | 1.030 ± 0.038 | 46 ± 1.5 | 32.1 ± 0.2 | 35 ± 1.8 | 2.2 ± 0.3 | 19 ± 0.4 |
| Δflv4 | 1.384 ± 0.097 | 30 ± 1.8 | 35.2 ± 2.6 | 36 ± 2.1 | 1.3 ± 0.2 | 34 ± 0.8 |
Measurements were made in the absence and presence of the electron transfer inhibitor DBMIB that binds to the Q0 site of the Cytb6f complex. The fitting parameters of fluorescence relaxation curves are presented as a mean ± se from three independent cultures and two to three measurements from each culture.