Figure 3.

Ph-4CL1 and Ph-CNL Belong to Distantly Related Subfamilies within the AAE Superfamily Defined by Shockey and Browse (2011).

A nonexhaustive BLASTp search was performed to retrieve additional Ph-4CL1 and Ph-CNL orthologs. Protein sequences were aligned using ClustalO (version 1.0.3; Sievers et al., 2011; see Supplemental Data Set 1 online). The resulting alignment was used to generate a neighbor-joining cladogram with the PHYLIP package (version 3.69; Felsenstein, 2009). Bootstrap values (1000 replicates) are indicated at branch points with ≥70% support. See Supplemental Table 1 online for sequence accession numbers. Species abbreviations: At, Arabidopsis; Cb, C. breweri; Gm, G. max; Nt, N. tabacum; Os, Oryza sativa; Ph, P. hybrida; Pp, P. patens; Ps, P. sitchensis; Pt, P. trichocharpa; Ri, R. idaeus; Sb, Sorghum bicolor; Zm, Zea mays.