Figure 6.

Subcellular Localization of Ph-CNL Protein.

Schematic diagram of the GFP fusion constructs is shown on the left, and the corresponding transient expression in Arabidopsis protoplasts detected by confocal laser scanning microscopy is shown on the right. GFP fluorescence is shown in green and chlorophyll autofluorescence in blue. Mitochondria were stained with MitoTracker-Red to distinguish them from peroxisomes and are shown in red with the exception of (E). In (E), YFP fluorescence of OPCL1 [3-oxo-2-(2′-[Z]-pentenyl)cyclopentane-1-octanoic acid (OPC-8:0) CoA Ligase1] used as a peroxisomal marker is shown in red. Merged panel shows combined fluorescence from GFP, mitochondria, chloroplasts, and peroxisomes. Numbers in the fusion constructs correspond to amino acids positions. ARL is the peroxisomal targeting signal found in Ph-CNL. For each construct, results of one of the two independent transformations are presented. Bars = 50 μm.