Table 1.
Kinetic Parameters of Ph-4CL1 and Ph-CNL Proteins
| Enzyme | Substrate (Acid) | Km (µM) | Vmax (pkat mg−1) | kcat (s−1) | kcat/Km (mM−1 s−1) |
|---|---|---|---|---|---|
| Ph-4CL1 | t-Cinnamic | 149.4 ± 13.7 | 14680 ± 409 | 0.847 ± 0.02 | 6.43 ± 0.86 |
| 4-Coumaric | 16.4 ± 3.0 | 25797 ± 1994 | 1.488 ± 0.12 | 84.32 ± 9.00 | |
| Caffeic | 47.8 ± 5.4 | 63200 ± 2020 | 3.645 ± 0.12 | 70.14 ± 5.95 | |
| Ferulic | 15.2 ± 2.7 | 41377 ± 879 | 2.39 ± 0.05 | 145.37 ± 27.21 | |
| Benzoic | 9008 ± 1795 | 95.9 ± 8.5 | 0.0007 ± 0.0001 | 0.0008 ± 0.0001 | |
| CoA | 9.9 ± 3.1 | 8451 ± 4017 | 0.487 ± 0.23 | 49.46 ± 6.51 | |
| Ph-CNL | t-Cinnamic | 285.7 ± 63.9 | 7401.7 ± 117.2 | 0.472 ± 0.01 | 1.82 ± 0.36 |
| 4-Coumaric | 550.4 ± 127.5 | 3890.7 ± 460.1 | 0.248 ± 0.03 | 0.512 ± 0.14 | |
| Caffeic | 1042.4 ± 95.9 | 3742.7 ± 3.6 | 0.238 ± 0 | 0.232 ± 0.02 | |
| Ferulic | n.d. | n.d. | n.d. | n.d. | |
| Benzoic | n.d. | n.d. | n.d. | n.d. | |
| CoA | 775.2 ± 34.3 | 7179.7 ± 126.8 | 0.458 ± 0.01 | 0.607 ± 0.06 |
All values represent mean ± se, n = 3; n.d., not determined, below detection limit.