Figure 9.
Immunoblot Analysis of C. intestinalis and Protist Actin Expression in act8-2 act7-4 Plants.
(A) Reactivity of MAbGEa with C. intestinalis cytoplasmic-like actin ACTB1 (CiB) and diverse protist actins. Ac, A. castellanii; Mb, M. brevicollis; Cs, C. scutata. About 1 μg of crude recombinant actin was loaded per lane.
(B) Expression of Ci-ACTB1 in act8-2 act7-4 plants. Three independent lines are shown. WT, the wild type.
(C) Expression of protist actin proteins in act8-2 act7-4 plants. Two independent lines are shown for each protist actin examined.