Figure 12.

RM Size and Uptake of Filipin-Sterols Are Reduced in Transgenic amiRNA Arabidopsis Knockdown Plants.

(A) RMs of 3-d-old wild-type (left) and amiRNA15-5 (right) seedlings were fixed, embedded, and stained with toluidine blue. Longitudinal sections were examined under a bright-field light microscope. Top black arrow indicates the transition zone between meristem and elongation differentiation zone; bottom arrow marks the quiescent center of the RM.

(B) Filipin-sterol fluorescence detected in wild-type (WT) Arabidopsis root cells. Pseudocolor images show the fluorescence intensity of filipin (right). (Color code bar indicates the relative intensities.)

(C) Filipin-sterol fluorescence detected in amiRNA Arabidopsis knockdown root cells. Pseudocolor images show the fluorescence intensity of filipin (right). (Color code bar indicates the relative intensities.)

Bars = 20 μm.