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. 2012 May 15;24(5):2105–2122. doi: 10.1105/tpc.112.095695

Figure 7.

Figure 7.

GFP-Flot1 and FM4-64 Puncta Are Often Colocalized at the Plasma Membrane of the Root Epidermal Cells.

(A) VA-TIRFM image of a root epidermal cell expressing GFP-Flot1 treated with FM4-64. White arrows indicate examples of colocalized puncta.

(B) Colocalization histogram of a plot for each pixel according to its red (x axis) and green (y axis) intensity. These data were analyzed using Manders coefficients.

Bars = 5 μm.