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. 2012 May 15;24(5):2105–2122. doi: 10.1105/tpc.112.095695

Figure 8.

Figure 8.

Flot1-Positive Puncta Derived from the Plasma Membrane Can Be Differentiated from CLC-Positive Puncta by their Dynamic Characteristics.

(A) and (B) VA-TIRFM image of a root cell expressing GFP-Flot1 and CLC-mOrange.

(C) Number of GFP-Flot1 and CLC-mOrange labeled puncta observed in VA-TIRFM movies of transgenic double-labeled Arabidopsis root epidermal cells.

(D) Histogram of mean velocity of tracked GFP-Flot1 and CLC-mOrange–labeled puncta in VA-TIRFM movies of transgenic double-labeled Arabidopsis root epidermal cells.

(E) and (F) Distribution of diffusion coefficients of tracked GFP-Flot1 and CLC-mOrange–labeled puncta in VA-TIRFM movies of transgenic double-labeled Arabidopsis root epidermal cells.

Bars = 10 μm.