ROS and Fe Accumulation, Suppression of Catalase, and Late Greening Phenotypes in Arabidopsis Seedlings with Suppressed Expression of NEET.
(A)
ROS accumulation in seedlings with wild-type (WT) or suppressed expression of NEET. ROS levels were measured in seedlings grown under controlled conditions or incubated with 0.1 µM of the ROS-generating compound paraquat.
(B) Protein blot analyses of different Fe- and ROS-associated proteins in seedlings with wild-type or suppressed expression of NEET.
(C) Fe content of 2-week-old plants with wild-type or suppressed expression of NEET. Plants grown under controlled conditions and fertilized with 1× or 100× Fe.
(D) Time course of greening in etiolated seedlings with wild-type or suppressed expression of NEET.