Figure 5.

Upregulation of PHO1 at the Protein Level in pho2.

(A) Immunoblot analysis of PHO1 in the root of 12-d-old wild-type (WT), pho1-2, and pho2 seedlings under +Pi and −Pi (6 d of Pi deficiency) conditions. The bottom panel shows protein staining on the membrane.

(B) Quantitative RT-PCR analysis of PHO1 expression in the root of 12-d-old wild-type, pho1-2, and pho2 seedlings under +Pi conditions. The value is presented as the fold change relative to the expression of wild-type plants. Error bars represent se (n = 3). Data significantly different from the wild-type controls are indicated (**P < 0.01; Student’s t test).

(C) The expression level of PHO1 protein in the root of 13-d-old wild-type and pho2 seedlings over 72 h of Pi recovery (250 µM KH₂PO₄) following 8 d of Pi deficiency. Actin is used as a loading control.