Figure 8.

The N-Terminal Part of PHO1 Is Required for PHO2 Activity-Mediated Degradation of PHO1.

Immunoblot analyses of the expression of full-length (A) or truncated PHO1 variants (B) when coexpressed with the functional PHO2^WT (35S:PHO2; in [A] and [B]) or catalytically defective PHO2^C748A (35S:PHO2^C748A; in [A]) in tobacco leaves. Coinfiltration of the 35S:GFP construct was used as an internal control. Note that ++ denotes a 10-fold increase in volume of infiltration relative to +. WT, the wild type.