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. 2012 May 25;24(5):2168–2183. doi: 10.1105/tpc.112.096636

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Figure 9. — Subcellular Localization of PHO1.

(A) Analyses of subcellular localization of PHO1-YFP (35S:gPHO1insYFP) using the ER, Golgi, trans-Golgi network (TGN), and endosomal markers in Arabidopsis protoplasts. Bars = 10 μm.

(B) Cellular and subcellular localization of PHO1-YFP (PHO1_pro:gPHO1insYFP) in the Arabidopsis roots of a 5-d-old wild-type transgenic seedling under +Pi conditions. Bars = 20 µm.

(C) The majority of PHO1 is detected in the EM-enriched fraction. PIP1;1/2/3 and PHF1 are used as PM- and EM-localized markers, respectively. The bottom panel shows the protein staining on the membrane. WT, the wild type.