Figure 4.

H₂O₂ Effects on GAPC and PLDδ Activities.

(A) RT-PCR detection of GAPC1 and GAPC2 expression in the leaves of wild-type (WT) and mutant plants. 18S rRNA was a control confirming the synthesis of cDNA.

(B) GAPDH activity in the total protein extracted from the leaves of wild-type and mutant plants.

(C) GAPDH activity using protein extracted from protoplasts after 1 mM H₂O₂ treatment.

(D) H₂O₂-promoted PA production in protoplasts. Values are means ± se (n = 3). Different letters mark significant differences from each other (ANOVA, P < 0.05).