Figure 1.

Suppression of mkk1 mkk2 Mutant Phenotypes by summ1-1 and summ1-2.

(A) Morphology of the wild type (WT), mkk1 mkk2 (mkk1/2), summ1-1 mkk1 mkk2, and summ1-2 mkk1 mkk2. The photograph shows 4-week-old soil-grown plants.

(B) and (C) Trypan blue (B) and DAB (C) staining of wild-type, mkk1 mkk2 (mkk1/2), summ1-1 mkk1 mkk2, and summ1-2 mkk1 mkk2 seedlings.

(D) and (E) PR1 (D) and PR2 (E) expression in wild-type, mkk1 mkk2 (mkk1/2), summ1-1 mkk1 mkk2, and summ1-2 mkk1 mkk2 seedlings. Values were normalized to the expression of ACTIN1. Error bars represent ±sd of three replicates.

(F) Growth of H.a. Noco2 on the wild type, mkk1 mkk2 (mkk1/2), summ1-1 mkk1 mkk2, and summ1-2 mkk1 mkk2. Three-week-old seedlings were sprayed with H.a. Noco2 spores (5 × 10⁴ spores/mL). Infections were scored 7 d after inoculation by counting the number of resuspended conidia spores per gram of leaf samples. Error bars represent ±sd of three replicates. This experiment was repeated three times with similar results.