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. 2001 May 29;98(12):6759–6764. doi: 10.1073/pnas.121569298

Figure 2.

Figure 2

Generation and analysis of the transgenic mice. (A) Structure of the transgenes. The Prm1-SB-β-globin transgene is a fragment of plasmid pRP1345. A fragment of the mouse protamine 1 (Prm1) promoter was cloned in front of the ORF encoding the SB transposase, followed by the rabbit β-globin polyadenylation signal. The SB/neo transgene is a fragment of plasmid pT/neo. It has an SV40-G418R cassette in between the inverted repeats of the transposon. The fragments were injected into different FVB/N oocytes. Five founders (designated as lines A–E) transmitting the Prm1-SB transgene to their offspring were obtained, ranging in copy number from 1 to 5. One line carrying a single copy of the complete SB/neo transposon was obtained (line 1). (B) RT-PCR analysis of the expression of SB transposase mRNA in several tissues, including brain (B), liver (L), skeletal muscle (M), testis (T) and kidney (K) of wild-type and transgenic mice (shown are lines B and E) using primers indicated in A by arrows.