Table 2.
Transposon insertion sites of SB/neo in the mouse genome
| Mouse | Left flank* | SB/neo | Right flank* | Chr† | Linked to† |
|---|---|---|---|---|---|
| Founder | AACACTGATACCCTA | cagt … actg | TAGGGGATCCTCTAG | 5 | D5Mit242 |
| 1576 | CCTAGGGACTAGCTA | cagt … actg | TATAACAGATGTCAA | 12 | D12Mit190 |
| 1579 | cagt … actg | TATCTCATGATGATG | n.d. | ||
| 1633 | CATCTCTCCAATATA | cagt … actg | TAGCTCTAGATGGTC | 5 | D5Mit237 |
| 1657 | cagt … actg | TATGTGAGTTGGGTG | 5 | D5Mit430 | |
| 1680 | cagt … actg | TATATATAGGTATAT | 5 | D5Mit242 | |
| 1682 | GTACCCCCTCACCTA | cagt … actg | TATTAAATCTTTTAA | 5 | D5Mit237 |
| 1688 | CTACAGACAAATATA | cagt … actg | TAGGTTTCACTCTTA | 5 | D5Mit237 |
| 1704 | GATCAGTTA | cagt … actg | n.d. | ||
| 1725 | cagt … actg | TATATC | n.d. | ||
| 1734 | CCTCCGGTAGTACTA | cagt … actg | TATGTCCAATTTTCT | n.d. | |
| 1775 | TATTTATCAGATATA | cagt … actg | TAAGTAAGTCTTCTA | 5 | D5Mit237 |
| 1797 | TATGTTCTTTATGTA | cagt … actg | n.d. | ||
| 1814 | TGCCTAATAAACATA | cagt … actg | TAACACTGCTCCGTG | 5 | D5Mit414 |
| 1818 | cagt … actg | TAGCTCCAGGGCATT | n.d. | ||
| 1820 | AATGGCTAATACATA | cagt … actg | 5 | D5Mit237 | |
| 1835 | GCAGCCCACTACCTA | cagt … actg | TAGTTCTCCCCCTTC | 5 | D5Mit242 |
| 1836 | cagt … actg | TATGTGTAAGAAAAC | 7 | D7Mit161 | |
| 1842 | cagt … actg | TAGGTCTGTTCCATG | 5 | D5Mit362 |
n.d., not determined.
*
The sequences flanking SB/neo (in capitals) were determined by using a transposon display method (24).
†
Using the T31 mouse/hamster radiation hybrid panel the insertion sites were mapped. The chromosome and the marker with the highest logarithm of odds score are indicated. Six insertions could not be mapped due to either the lack of sufficient flanking sequence or the presence of multiple copies of the insertion site sequence in the mouse genome. blast (51) searches showed that both in mouse 1579 and in mouse 1734 SB/neo had integrated into an L1 retrotransposon.