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. 2012 Apr 1;2(2):89–97. doi: 10.4161/bact.21363

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Copyright © 2012 Landes Bioscience

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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graphic file with name bact-2-89-g4.jpg — Figure 4. Localization of ΦS9 and ΦS63 attachment sites attB in the C. perfringens genome. (A) Schematic representation of the integration of ΦS9 into C. perfringens genomic DNA (sequence from ATCC 13124). (B) Integration of ΦS63 into C. perfringens (partial genome sequence of S63 determined in this work). (C) Location of ΦS9 in the C. perfringens genome. Core sequence (11 nt) used in recombination is boxed. P, promoter region for cpf_0925 homolog. Primer binding sites are indicated by arrows. (D) Diagram showing the location of ΦS63 in the C. perfringens genome. Core sequence (10 nt) for recombination is boxed. Primer binding sites are indicated by arrows. (E) PCR-based confirmation of the ΦS9 attachment site, using C. perfringens S9 and S9ΔΦS9 genomic DNA as templates, and (F) the ΦS63 attachment site, using C. perfringens S63 genomic DNA as template. Primer binding sites are indicated.