Figure 1. MscS Family Members in Organelles and Intracellular Pathogens. Thirty MscS homologs from 14 species of bacteria and 5 species of plants were identified by Aramemnon cluster analysis (http://aramemnon.botanik.uni-koeln.de/index.ep), previously published analyses,^19,25 or inclusion in the Ortho MCL database (http://orthomcl.org/cgi-bin/OrthoMclWeb.cgi). The MscS-like region of each protein sequence was determined by InterProScan,²⁶ and aligned using ClustalX²⁷ with a multiple sequence alignment gap opening penalty of 3.0 and an extension penalty of 1.8. A total of 326 positions, including gaps, were present in the final data set. Evolutionary relationships were inferred by Neighbor-Joining²⁸ and JTT distance matrix methods²⁹ using MEGA5 software. The percentage of trees in which the associated taxa clustered together in the bootstrap test (n = 2000 replicates) are shown next to the branches.³⁰ Only significant bootstrap values (greater than 75%) are shown, and branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. Scale bar, 0.2 amino acid substitutions per site.