Figure 4. Amino acid sequence and structural bioinformatics analysis of the LIR-MER region. The MER (underlined, starting at 70), as defined by N-terminal truncation and internal deletion mutants overlaps a region of predicted α-helical (H) structure. The algorithm produces a reliability index (REL IN), on a scale of 0–9. Nine is the highest degree of reliability. The BNIP3L LC3-interaction region (LIR)(underlined, starting at 35), overlaps a predicted region of β-strand (E). PredictProtein secondary structure, PP SEC.