Table 1.
Flow cytometric analysis of lymphocyte infiltration and cell activation status in control and CD1d-deficient NOD mice
| Mice | n | TCRαβ + DX5, % | CD4+
|
CD8+
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| CD25, % | CD44, % | CD62L, % | CCR4, % | CD25, % | CD44, % | CD62L, % | CCR4, % | |||
| Spleens | ||||||||||
| CD1d+/+ | 5 | 4.1 ± 0.2 | 7.3 ± 1.5 | 20.2 ± 4.9 | 66.3 ± 4.5 | 38.2 ± 6.6 | 5.2 ± 1.5 | 17.5 ± 2.5 | 68.7 ± 2.5 | 13.7 ± 3.4 |
| CD1d+/− | 8 | 2.6 ± 0.2 | 6.8 ± 2.7 | 17.3 ± 1.2 | 78.5 ± 2.7 | 35.0 ± 7.2 | 7.1 ± 1.7 | 16.6 ± 2.0 | 65.9 ± 1.0 | 15.4 ± 5.0 |
| CD1d−/− | 8 | 0.1 ± 0.0* | 8.2 ± 1.3 | 25.4 ± 3.0 | 74.2 ± 2.2 | 42.3 ± 3.3 | 6.2 ± 1.1 | 28.0 ± 2.7 | 72.2 ± 2.3 | 15.0 ± 7.1 |
| Pancreatic lymph nodes | ||||||||||
| CD1d+/+ | 5 | 3.6 ± 0.1 | 4.3 ± 0.2 | 31.5 ± 5.1 | 57.4 ± 6.5 | 47.0 ± 2.8 | 4.1 ± 0.5 | 21.0 ± 2.3 | 53.22 ± 5.05 | 14.5 ± 6.0 |
| CD1d+/− | 8 | 2.0 ± 0.1 | 4.2 ± 0.4 | 27.3 ± 3.8 | 66.5 ± 8.7 | 51.3 ± 8.0 | 6.3 ± 1.0 | 26.6 ± 1.8 | 60.00 ± 6.31 | 18.3 ± 6.1 |
| CD1d−/− | 8 | 0.2 ± 0.0* | 5.2 ± 0.3 | 30.2 ± 2.9 | 68.2 ± 3.4 | 58.2 ± 4.2 | 7.2 ± 0.2 | 32.5 ± 4.7 | 77.05 ± 5.60 | 20.2 ± 4.4 |
| Pancreata | ||||||||||
| CD1d+/+ | 5 | 5.5 ± 0.4 | 3.8 ± 0.6 | 23.7 ± 2.0 | 50.3 ± 2.4 | 45.0 ± 8.2 | 5.2 ± 1.0 | 18.2 ± 1.4 | 53.2 ± 5.0 | 18.4 ± 6.0 |
| CD1d+/− | 8 | 2.2 ± 0.2 | 4.1 ± 0.4 | 29.6 ± 2.8 | 62.2 ± 2.1 | 52.5 ± 6.3 | 7.0 ± 3.2 | 29.3 ± 2.8 | 40.0 ± 3.1 | 25.2 ± 4.5 |
| CD1d−/− | 8 | 0.5 ± 0.8* | 8.7 ± 0.6* | 38.7 ± 1.2* | 55.5 ± 6.1 | 77.2 ± 4.4* | 14.2 ± 2* | 32.5 ± 5* | 59.2 ± 6.2 | 31.0 ± 4* |
Mononuclear cells were isolated from spleens, pancreatic lymph nodes, and pancreata and labeled with mAbs as indicated. Data (mean ± SD) shown in the table are derived from 8-week-old female mice. *, P < 0.05, ANOVA.