Table 3.
Flow cytometric analysis of Vβ4+ tg cell infiltration and activation status in then CD1d−/− and CD1d+/+ BDC2.5 mice
| Mice | n | Spleen
|
Pancreatic
lymph nodes
|
Pancreata
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CD25, % | CD44, % | CD62L, % | CD25, % | CD44, % | CD62L, % | CD25, % | CD44, % | CD62L, % | CCR4, % | ||
| CD1d+/+ BDC2.5 | 4 | 6.2 ± 0.3 | 19.8 ± 1.3 | 79.4 ± 4.5 | 5.28 ± 1.21 | 22.3 ± 3.2 | 66.7 ± 4.2 | 6.33 ± 1.1 | 25.4 ± 3.3 | 58.8 ± 4.3 | 68.8 ± 8.0 |
| CD1d−/− BDC2.5 | 4 | 6.8 ± 0.4 | 22.5 ± 2.0 | 77.5 ± 3.3 | 8.08 ± 2.04 | 38.1 ± 2.6 | 45.8 ± 2.2 | 13.4 ± 1.2* | 42.0 ± 3.1* | 44.5 ± 2.7 | 96.2 ± 4.3* |
Mononuclear cells were isolated from spleens, pancreatic lymph nodes, and pancreata and labeled with mAbs as indicated. Data (mean ± SD) shown in the table are double-positive population for Vδ4 and each of the indicated Abs, derived from 8-week-old female mice. *, P < 0.05, ANOVA.