Figure 4. Deletions and rearrangements with endpoints in the supF gene and telomeric repeats. The supF gene with borders marked by “|,” telomeric or scrambled sequence insert and the XhoI and EagI restriction sites are shown 5' to 3'. Bases marked in grey are alterations in the tRNA acceptor stem. End points of deletions or rearrangements are marked by an open arrow head (shCTRL) cells or a solid arrow head (shWRN) cells. Deleted or altered sequences are all 3' to arrowheads unless otherwise noted with an arrow. The 5' end of the mature tRNA (*G) is marked as position 99 following the traditional nomenclature.