Figure 2. The SAC inhibits the MEN during metaphase. (A) and (B) Cells expressing the nnf1–17 allele in combination with mutations affecting Dbf2-dependent phosphorylation of Kar9. Cells coexpressed Kar9-YFP and CFP-Tub1 and were either MAD2 or mad2∆, as well as CLB5 or clb5∆. All quantifications were performed as in Figure 1C, cells were shifted to 37°C for 50 min before imaging. Stars indicate p-values obtained from one-way ANOVA comparing “strong asymmetry” (three clones with total n > 130). (C) Analysis of Kar9 asymmetry in FEAR mutant cells at 22°C as in (A).