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. 2012 Aug 15;11(16):3117–3131. doi: 10.4161/cc.21505

graphic file with name cc-11-3117-g2.jpg

Figure 2. Functional analisys of chimeric cyclin 4. (A) Diagram of chimeric cyclin 4. All cyclins are HA-epitope tagged at the C terminus. Expression is driven by the promoter corresponding to the cyclin at the N-terminal end. (B) Cell size distribution in exponentially growing cultures of the cln1 cln2 mutant strain (JCY847) transformed with an empty vector or a centromeric plasmid containing the CLN1, CLN2 or CHIMERA4 gene. (C) 10-fold serial dilutions from exponentially growing cultures of the cln2 mutant strain (JCY846) transformed with an empty vector or a centromeric plasmid containing the CLN1, CLN2 or CHIMERA4 gene were spotted onto YPD medium and YPD medium supplemented with 25 μM latrunculin B and incubated at 28° for 3 d. (D) Cells of the swi4ts swi6 mutant strain (K2003) transformed with an empty vector or a centromeric plasmid containing the CLN1, CLN2 or CHIMERA4 gene were streaked onto YPD plates and incubated at 37° for 3 d. (E) Exponentially growing cultures of the cdc28ts cln1 cln2 (JCY1048) and the cln1 cln2 (JCY847) mutant strains transformed with a centromeric plasmid containing the CLN1, CLN2 or CHIMERA4 gene were arrested at the G1 phase by incubation at 37° or in the presence of 5 μg/mL α-factor for 3 h, respectively. The budding index was determined at the indicated time after release from cell cycle arrest.