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Total ng of HBsAg produced by transfection of pCMV-HA alone, wild-type vector 1 alone or with mutation combinations M103I+K122R, M103I+G145A, K122R+G145A, or M103I+K122R+G145A, were calculated for supernatants (A,C) and cell lysates (B,D) from Huh7 (A,B) and HepG2 (C,D) cells in duplicate and compared for days 3 (grey bars), 5 (white bars), and 7 (black bars) post-transfection. * p<0.05; ** p<0.01; + p<0.001; ++ p<0.0001.
