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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Viral Hepat. 2012 Jun 4;19(10):716–723. doi: 10.1111/j.1365-2893.2012.01595.x

Table 2. Genotype A HBsAg Expression Vectors.

Wild-type partial S ORF sequences were derived from 2 genotype A C-HBV infections and ligated into the pCMV-HA vector. The wild-type vectors served as positive controls and were the backbones into which mutations associated with O-HBV infection were introduced by site-directed mutagenesis. A total of 17 vectors were utilized in these experiments as listed above.

Genotype A HBsAg Expression Vectors
Wild-Type 1 Wild-Type 2
Wild-Type 1 + M103I mutation Wild-Type 2 + M103I mutation
Wild-Type 1 + K122R mutation Wild-Type 2 + K122R mutation
Wild-Type 1 + G145A mutation Wild-Type 2 + G145A mutation
Wild-Type 1 + M103I + K122R mutations Wild-Type 2 + M103I + K122R mutations
Wild-Type 1 + M103I + G145A mutations Wild-Type 2 + M103I + G145A mutations
Wild-Type 1 + K122R + G145A mutations Wild-Type 2 + K122R + G145A mutations
Wild-Type 1 + M103I + K122R + G145A mutations Wild-Type 2 + M103I + K122R + G145A mutations
pCMV-HA alone (negative control)