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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Viral Hepat. 2012 Jun 4;19(10):716–723. doi: 10.1111/j.1365-2893.2012.01595.x

Table 3. HBsAglysate:HBsAgsupernatant Ratios.

Ratios comparing lysate HBsAg to supernatant HBsAg (HBsAglysate:HBsAgsupernatant) were calculated at days 3, 5, and 7 post-transfection for all constructs with the wild-type 1 backbone in both Huh7 and HepG2 cells. Bold values indicate constructs with higher intracellular/lysate levels of HBsAg (values >1). UD – indicates undetectable levels of HBsAg (below the lower limit of detection for the ELISA, <0.5ng/mL) in the lysate and/or supernatant.

Day 3 Day 5 Day 7
Construct Huh7 HepG2 Huh7 HepG2 Huh7 HepG2
Wild Type 1 3.44 0.83 0.98 0.85 0.70 0.63
M103I 2.95 0.68 0.90 0.83 0.51 0.52
K122R 1.88 0.76 0.90 0.60 0.60 0.50
G145A UD/0.43 UD/0.29 1.27 UD/1.09 1.61 1.11
M103I+K122R 1.18 1.12 0.96 0.62 0.69 0.44
M103I+G145A 2.64 UD/0.17 1.09 UD/UD UD/0.85 UD/0.62
K122R+G145A UD/UD UD/UD UD/0.31 UD/0.35 UD/UD UD/UD
M103I+K122R+G145A UD/0.08 UD/UD UD/0.44 UD/0.26 UD/0.08 UD/UD
pCMV-HA UD/UD UD/UD UD/UD UD/UD UD/UD UD/UD