Table 1.
Isolate | Morphology | Biomass (CDW ing L-1)* |
Total lipid#(g g-1of CDW)* |
Lipid yield#( g L-1) |
||
---|---|---|---|---|---|---|
Bead beater | Liquid nitrogen | Bead beater | Liquid nitrogen | |||
IBB B2 |
Mycelial |
4.27 ± 0.1a |
0.11 ± 0.02ab |
0.25 ± 0.01a |
0.47 ± 0.02a |
1.07 ± 0.1a |
IBB F14 |
Mycelial |
4.14 ± 0.3a |
0.15 ± 0.02ab |
0.28 ± 0.01a |
0.62 ± 0.01a |
1.16 ± 0.3ab |
IBB G4 |
Mycelial |
5.84 ± 0.5bc |
0.17 ± 0.04b |
0.30 ± 0.05a |
0.99 ± 0.02b |
1.75 ± 0.2bc |
IBB G5 |
Mycelial |
6.44 ± 0.3c |
0.09 ± 0.01a |
0.30 ± 0.03a |
0.57 ± 0.02a |
1.93 ± 0.2c |
IBB M1 | Mycelial | 5.52 ± 0.2b | 0.10 ± 0.01a | 0.54 ± 0.03b | 0.552 ± 0.3a | 2.98 ± 0.3d |
* CDW - cell dry weight determined after 72 h.
# Lipid extractions carried out by two different cell lysis techniques as mentioned in Methods.
The data are shown as the mean ± standard deviation (n = 3). a,b,c Mean values within a column with different superscript letters differ significantly and were determined statistically as mentioned in Methods (one way ANOVA, Tukey, p < 0.05). Separate analysis was done for each column.