Table 4.
Plasmid used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pFA6a-KanMX6 |
E. coli kanr gene under the control of Ashbya gossypii TEF promoter-driven |
[45] |
| pCNKanMX6 |
loxP flanking KanMX6 at SalI/BamHI and SacI/EcoRV sites |
This study |
| pTKURA3 |
loxP flanking S. cerevisiae URA3 gene |
This study |
| p423GPD |
2 μ, HIS3, PGPD1 |
[46] |
| p423TEF |
2 μ, HIS3, PTEF1 |
[46] |
| p423GPD-BDH1 |
S. cerevisiae BDH1 gene under the control of GPD1 promoter in p423GPD |
This study |
| p423TEF-BDH1 |
S. cerevisiae BDH1 gene under the control of TEF1 promoter in p423GPD |
This study |
| p423GPD-E.a.budC |
E. aerogenes KCTC 2190 budC gene under the control of GPD1 promoter in p423GPD |
This study |
| pESC-URA |
2 μ, URA3, PGAL10 PGAL1 |
Stratagene |
| pESC-LEU |
2 μ, LEU2, PGAL10 PGAL1 |
Stratagene |
| pESC-LEU-Cre |
P1 bacteriophage Cre recombinase under the control of GAL10 promoter |
This study |
| pCTP-URA |
2 μ, URA3, PTEF1 PPGK1 |
This study |
| pCTP-URA::TEF1p-alsS + PGK1p-budA | Bacillus subtilis alsS gene under the control of TEF1 promoter and E. aerogenes KCTC 2190 budA gene under the control of PGK1 promoter in pCTP-URA | This study |