Table 1.
Growth parameters of P. pastoris growing on glucose:methanol in chemostats cultures
| Strain | Glucose mmol/g CDW·h | Methanol mmol/g CDW·h | OUR mmol/g CDW·h | CER mmol/g CDW·h | Biomass mmol/g CDW·h | YX/S g CDW/Cmol | RQ | Lipase activity AU/gCDW |
|---|---|---|---|---|---|---|---|---|
|
X-33 control |
−0.76 ± 0.02 |
−1.15 ± 0.06 |
−2.95 ± 0.11 |
2.31 ± 0.11 |
3.40 ± 0.04 |
14.7 ± 0.3 |
0.78 ± 0.05 |
n.d. |
|
ROL 1-copy |
−0.85 ± 0.01 |
−1.29 ± 0.05 |
−3.71 ± 0.07 |
3.04 ± 0.06 |
3.32 ± 0.03 |
13.0 ± 0.3 |
0.82 ± 0.09 |
2504 ± 192 |
| ROL 2-copy | −0.87 ± 0.01 | −1.24 ± 0.09 | −3.66 ± 0.06 | 3.04 ± 0.04 | 3.39 ± 0.05 | 12.9 ± 0.1 | 0.83 ± 0.07 | 3490 ± 208 |
Reconciled measured substrates and products consumption or production rates at steady state conditions in each experiment. Substrate consumption, biomass and metabolites production rates for each strain. OUR, Oxygen Uptake Rate; CER, CO2 Exchange Rate; RQ Respiratory Quotient; n.d., not detectable. Consistency index h was below 7.81 for a redundancy of 3 (95% significance level) in all cases.