Table 4.
Amino acid composition of P. pastoris
| |
80% Glucose 20% Methanol |
100% Glucose |
|||
|---|---|---|---|---|---|
| % mol/mol | X-33 | ROL 1-copy | ROL 2-copy | X-33* | S. cerevisiae ** |
|
Arg |
8.15 |
7.67 |
6.45 |
7.04 |
3.86 |
|
Asp |
9.25 |
9.48 |
10.05 |
8.78 |
9.28 |
|
Thr |
6.15 |
6.06 |
6.46 |
5.88 |
5.57 |
|
Ser |
6.34 |
6.32 |
6.82 |
6.26 |
5.33 |
|
Glu |
15.76 |
15.44 |
13.23 |
17.81 |
15.48 |
|
Pro |
4.2 |
4.0 |
3.92 |
3.83 |
4.22 |
|
Gly |
7.80 |
7.53 |
7.86 |
6.86 |
8.89 |
|
Ala |
11.14 |
9.41 |
9.36 |
10.40 |
9.77 |
|
Val |
6.81 |
6.67 |
6.54 |
5.88 |
7.33 |
|
Cys |
0.17 |
0.19 |
0.13 |
0.15 |
0.14 |
|
Met |
0.78 |
0.75 |
0.62 |
0.79 |
1.14 |
|
Ile |
4.49 |
4.49 |
4.69 |
4.64 |
5.89 |
|
Leu |
7.50 |
7.45 |
8.34 |
6.96 |
8.01 |
|
Tyr |
2.28 |
2.19 |
2.51 |
2.16 |
1.96 |
|
Phe |
3.44 |
3.31 |
3.61 |
3.20 |
3.76 |
|
Orn |
0.68 |
0.32 |
0.26 |
1.04 |
0.24 |
|
Lys |
6.77 |
6.77 |
7.21 |
6.41 |
6.57 |
|
His |
2.10 |
1.94 |
1.95 |
1.89 |
1.93 |
| Trp | 1.00 | 1.00 | 1.00 | 1.40 | 1.96 |
* Data taken from [26]; ** Data taken from [27].
Amino acid composition of the whole cell extract for all the P. pastoris strains tested. Amino acid composition measured for the three different ROL gene dosages. Data given as percentage of each amino acid in the total pool of amino acids. The standard deviation of the analytical method was 3~5%.