Table 5.
Metabolic flux ratio (METAFoR) analysis results
| % Fraction of total pool | X-33 control | ROL 1- copy | ROL 2-copy | X-33 control* |
|---|---|---|---|---|
| |
Glucose:Methanol |
Glucose:Methanol |
Glucose:Methanol |
Glucose |
|
Pep from methanol |
45 ± 5 |
34 ± 9 |
24 ± 9 |
n.a. |
|
Pep from PPP (upper bound) |
14 ± 4 |
21 ± 8 |
24 ± 11 |
39 ± 9 |
| R5P from T3P and S7P (transketolase) |
77 ± 7 |
70 ± 1 |
83 ± 2 |
66 ± 2 |
| R5P from E4P (transaldolase) |
56 ± 1 |
57 ± 9 |
56 ± 4 |
40 ± 2 |
| Ser originating from Gly and C1-unit |
53 ± 3 |
55 ± 2 |
58 ± 1 |
62 ± 4 |
| Gly originating from CO2 and C1-unit |
7 ± 1 |
12 ± 1 |
8 ± 2 |
6 ± 4 |
| Pep originating from Oaacyt (PepCK) |
n.a. |
n.a. |
n.a. |
2 ± 5 |
| Oaamitoriginating from Pep | 49 ± 3 | 42 ± 12 | 36 ± 2 | n.a. |
*Data taken from [26].
METAFoR analysis results showing the origins of metabolic intermediates during growth of P. pastoris on glucose:methanol compared with those growing on glucose as the sole carbon source, n.a. (not available). Flux ratios used for MFA are highlighted in bold.