Figure 5.

Mechanical pain thresholds in animals with early-life (P10) nerve injury (S) or sham surgery H) tested in later life (P31). Mechanical pain thresholds were measured in minocycline (M) (40 mg/Kg daily ip injections) after SNI nerve injury compared to SNI saline (S) group and controls (H). n = 6 per experimental group; *: ANOVA, p < 0.001, Tukey, p < 0.05 B) Representative images of western-blot for phospho-p38 and phospho-JNK (pJNK) and the housekeeping gene Gapdh. Quantification was done by densitometry of p38 (C) and pJNK(D). *: n = 4 per experimental group; *: ANOVA, p < 0.001, Tukey, p < 0.05.