Table 1.
Experimental conditions used for validation
| Reference | Gene KOs | Media conditions |
|---|---|---|
| BioloMICS [16] |
– |
46 different carbon sources |
| Thevenieau,2007 [10] |
15 gene KOs |
YNBD, YNBO, YNBC10, YNBC16, YNBT |
| T van den Temple, 2000 [17] |
– |
Lactose, D-Galactose |
| Jardon, 2008 [18] |
FBP1 |
YNBD, Ethanol, Glycerol, Acetate |
| Flores, 2005 [19] |
PYC1, ICL1 |
YNBD, Ethanol, Aspartate, Glutamate |
| Yamagami, 2001 [20] |
PAT1 |
YNBC10, YNBD, Glycerol |
| Haddouche (PC) [21] |
ACL1 |
YNBD, YNBO |
| Kabran, 2010 [22] |
ICL1, MLS1, CIT2 |
Acetate, YNBO, YNBD |
| Beopoulos, 2008 [23] |
GUT2, POX1-6 |
YNBD, Glycerol, YNBO |
| Jiménez-Bremont, 2001 [24] |
OCD1 |
YNBD, YNBD + putrescine |
| Cheon, 2003 [25] | TRP1 | YNBD, YNBD + tryptophane |
Literature sources used for validation of the Y. lipolytica model. Overall, 60 different media conditions were tested. Gene knockouts were assessed for 29 different Y. lipolytica gene loci, in 152different experiments. Only those cases where evident growth/no growth was observed were included in this analysis.