Table 1.
Colchicine treatment | Transferred embryos | Regeneration rate | Green plants [%] | Survival rate [%] |
---|---|---|---|---|
24 h - 0 mM |
330 |
42.4 a |
54.3 a |
85.5 a |
24 h - 0.3 mM |
240 |
39.2 a |
61.7 ab |
81.0 ab |
24 h - 1 mM |
240 |
41.7 a |
72.0 ab |
83.3 ab |
24 h - 5 mM |
180 |
41.7 a |
65.3 ab |
71.4 ab |
48 h - 0 mM |
210 |
43.3 a |
75.8 b |
62.3 bc |
48 h - 0.3 mM |
240 |
47.1 a |
59.3 ab |
82.1 ab |
48 h - 1 mM |
240 |
47.1 a |
69.0 ab |
43.6 cd |
48 h - 5 mM |
120 |
24.2 b |
41.4 a |
50.0 ab |
72 h - 0 mM |
210 |
42.4 a |
48.3 a |
60.5 b |
72 h - 0.3 mM |
240 |
43.8 a |
69.5 ab |
68.5 ab |
72 h - 1 mM |
240 |
33.3 a |
62.5 ab |
64.0 ab |
72 h - 5 mM |
270 |
26.3 b |
71.8 ab |
62.7 ab |
in vivo | 835 | 58.8 c | 70.9 b | 44.0 d |
Survival rate refers to the rate of plants that survived the transfer from culture medium to soil and grew to maturity. Means of columns followed by different letters indicate significant differences at the α = 0.05 level.