Figure 1. The kinin B₁R is constitutively expressed in WAT and downregulated with obesity.

A: Kinin B₁R mRNA expression was quantified in different organs of male C57Bl/6 mice (n = 5) using TaqMan real-time PCR. Data are expressed as mean±SEM of the 2^−ΔCt parameter, which represents the relative expression of B₁R mRNA in relation to β-actin mRNA. WAT, white adipose tissue; ND, non-detectable; a.u., arbitrary units. B: Effects of acute treatment with the B₁R agonist des-Arg⁹-bradykinin (DBK), the B₂ receptor agonist bradykinin (BK) or DBK in the presence of the B₁R antagonist [Leu⁸]-DBK on the extracellular acidification rate of isolated adipocytes from wild type (WT) and B₁ knockout (B₁ ^−/−) mice (n = 5 animals per group) measured using the Cytosensor system. C: Representative RT-PCR showing expression of components of the kallikrein kinin system kallikrein in WAT, lung, and heart of C57Bl/6 male (n = 3). KK, tissue kallikrein; CPM, carboxypeptidase M; B₂, kinin B₂ receptor. C-, negative control. D: C57BL/6 mice were submitted to HFD for 9 weeks (n = 5 per group). Kinin B₁R expression was quantified in WAT by real time PCR. Results are mean ± SEM. *, P<0.05.