Figure 3. Activation of lipid utilizing pathways in muscles of HFD-fed control and mGHRKO mice in response to 24 h fasting challenge.

14-wk HFD-fed control and mGHRKO mice were subjected to a 24 h fasting challenge. (A–G). mRNA expression of indicated genes in quadriceps muscles of fed, and 24 h fasted mice (n = 6–8/group). Each sample was probed three times and gene expression was normalized to β-actin and represented as a fold change when compared to the fed mice for each genotype. H. Phosphorylation of AMPKα at Thr¹⁷² was determined in protein lysates isolated from quadriceps muscle of fed and fasted control and mGHRKO mice. Levels of phosphorylated AMPKα was normalized to total protein content of AMPKα and represented as a fold changed compared to fed mice of each genotype. β- tubulin was used to determine equal loading (n = 6–8/group; representative blots are provided). All values are represented as mean ± S.E.M. *−p≤0.05 versus respective fed group, #−p≤0.05 fasted control versus fasted mGHRKO, Two-way ANOVA.