Figure 5. Lipid handling the control and mGHRKO mice in response to 24 h fasting.

14-wk HFD-fed control and mGHRKO mice were subjected to a 24 h fasting challenge. A. Muscle TG content in the gastrocnemius was determined in the fed and fasted mice (n = 6–8/group). (B–F). Western blots were performed to determine total protein content of ATGL and HSL, and the phosphorylated levels of HSL at residues Ser⁶⁶⁰ and Ser⁵⁶³ in the quadriceps muscle. β-tubulin was used to determine equal loading. Levels of phosphorylated HSL at Ser⁶⁶⁰ and Ser⁵⁶³ were normalized to total HSL content, while total protein content of ATGL and HSL were normalized to β-tubulin. The data are represented as a fold change compared to the fed state levels for each genotype (n = 6–8/group; representative blots are provided). All values are represented as mean ± S.E.M. *− p≤0.05 versus respective fed group, One-way ANOVA.