Figure 5. Effect of exogenous and endogenous IL-10 on fold changes in cytokine and chemokine levels as produced by macrophages from Lyme disease-resistant and -susceptible mice.

BMDM were stimulated with live Bb or L-OspA in the presence or absence of exogenous or α-IL-10 (or its isotype control) Ab as described in Figs. 3 and 4. Cell-free supernatants were harvested from cultures at 24 h and protein determinations were made by multiplex ELISA. Fold decrease (A) was calculated as stimulant/stimulant exposed to exogenous IL-10. Fold increase (B) was calculated as stimulant + α IL-10 Ab/stimulant+isotype control Ab. A one-way ANOVA followed by a Bonferroni post-hoc test in conjugation with two tailed Mann Whitney test was used for data analyses. Significance was established at P<0.001 = ***, P<0.01 = ** and P<0.05 = *. Each symbol represents fold change for a specific cytokine or chemokine. Each fold change is representative of two independent experiments.